Advanced Techniques of Bovine Semen Analysis

Interpretation of Basic Semen Analysis and Advanced Semen Testing

A consensus exists that the basic semen analysis is the most important tool in male fertility investigation. In the last two decades, researchers and clinicians alike have relied on the World Health Organization (WHO) criteria for the interpretation of basic semen analysis. Nevertheless, the criteria for what constitutes a normal semen analysis remain controversial. Although a single test such ...

Glutatione peroxidase in bovine semen.

The roles ofselenium (Se) and glutathione peroxidase in reproductive function are poorly understood, but it is possible that they may be important for normal reproduction in the male. In rats fed a Se-deficient diet, the testes accumulated and retained more 75Se than did other tissues 1 week after injection (Brown & Burk, 1972; Burk, Brown, Seely & Scaief, 1972). Autoradiographic studies (Brown...

Advanced Techniques for Scene Analysis

Detection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

The amino acid composition of bovine semen.

Very few data are available concerning the amino acid content of mammalian sperm. The early work by Kossel (1) on the protamines of fish sperm and on the histones was primarily concerned with an attempt to elucidate the structure of the basic proteins. The basic proteins are, in general, characterized by a high arginine content. Zittle and O’Dell (2) have determined the methionine content of wh...